![]() We then designed a small-molecule inhibitor that efficiently blocks HtrA activity, E-cadherin cleavage and intercellular entry of H. Automated tissue resistance measurements up to 20k can be performed on 24- or 96-well HTS microplates. pylori designed to access the intercellular space. The REMS AutoSampler automates TEER measurements previously made with WPI’s EVOM2 Epithelial Voltohmmeter. ![]() ![]() E-cadherin shedding disrupts epithelial barrier functions allowing H. Here, we describe an entirely new function of HtrA and identify it as a new secreted virulence factor from Helicobacter pylori, which cleaves the ectodomain of the cell-adhesion protein E-cadherin. Mammalian and prokaryotic high-temperature requirement A (HtrA) proteins are chaperones and serine proteases with important roles in protein quality control. The Millicell-ERS (Electrical Resistance System) reliably measures membrane potential & resistance of epithelial cells in culture. Measurement of Transepithelial Electrical Resistance (TEER), Cell Monolayer Health Assessment, Quantitative Measurement of Confluence Widely cited and recommended in epithelial cell culture protocols Measurements do not compromise sterility or damage cells on the membrane EVOM was the first instrument designed to perform routine Trans Epithelial Electrical Resistance (TEER) measurement in tissue culture research. Epithelial cells grown alone or in coculture with stromal cells were treated with Oe (2). Portable instrument that fits into laminar flow hoods Transepithelial resistance (TER) was monitored with an EVOM voltohmmeter to determine monolayer polarity and integrity. Accessories include a replacement (fixed) electrode set, adjustable electrode set and a specialized electrode set for use with Millicell®-96 cell culture insert plates. Preparation of cell culture should be done under BSL-2 conditions and handling of inactivated viral samples from BSL-3 should be done under BSL-2 conditions with appropriate precautions. Due to the small size of the electrodes, you can easily measure transepithelial voltage and the resistance of cells grown on microporous membranes. TEER measurement for ensuring epithelial integrity is performed using an EVOM voltohmmeter device with STX2 electrodes. A silver/silver chloride (Ag/AgCl) pellet on each electrode tip measures voltage. This device qualitatively measures cell monolayer health and quantitatively measures cell confluence. The Millicell® ERS-2 (Electrical Resistance System) reliably measures membrane potential and resistance of epithelial cells in culture.
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